HeLa cells were subjected to SDS PAGE followed by western blot with FNab04683(LMNB1 antibody) at dilution of 1:10000
IP Result of anti-Lamin B1 (IP: FNab04683, 4ug; Detection: FNab04683 1:40000) with HeLa cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using FNab04683(Lamin B1 antibody) at dilution of 1:1000
Background
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.